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For differentiation, 5 10 5 iPSCs per well were seeded in a CELLSTAR6-well plate (Greiner Bio-one) in E6-medium supplemented with 50 ng/mL hbFGF, 1 ng/mL hTGFb1, 50 ng/mL human vascular endothelial growth factor (hVEGF) and human bone morphogenetic protein 4 (hBMP4), 20 ng/mL human stem cell factor (hSCF) (all PeproTech) and 10 M RI and placed on an orbital shaker (OMNI Life Science) at 70 rpm