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A Waters 2777c UPLC system (Waters, USA) coupled with a Q Exactive HF high-resolution mass spectrometer (Thermo Fisher Scientific) was utilized for the isolation and identification of metabolites

Hence, we are interested to identify multiple biomarkers to evaluate the skin irritancy potential of various metallic compounds, specifically, copper compounds

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sPLA 2 could metabolize phospholipids in platelets, erythrocytes, and endothelial cells to produce arachidonic acid and lysophospholipids [349]

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