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A patient managed with NSAIDs and "watchful waiting" for presumed OA can lose that window entirely

After the membranes were incubated in blocking buffer at room temperature for 2 h, they were washed 3 times in tris-buffered saline and tween 20 (TBST) solution, 5 min each, and then incubated with primary antibodies overnight at 4 C

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