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Description

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To favor the recombination event and recovery of recombinants, the transformation mixtures were incubated overnight in liquid medium without antibiotic and in the presence of AdoCbl, since MetH requires B12 as cofactor

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The more NNMT activity, the more your NAD+ precursors get converted into a form your cells can't use

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In research models, this inhibition indirectly preserves NAD by reducing methylation of nicotinamide, and also maintains SAM levels an effect precursors do not address

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