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The desalted sample was evaporated to dryness in a speedvac and then resuspended in 10 L Buffer A and analyzed by mass-spectrometry using the following LC-MS gradient: 5% buffer B in buffer A from 0-15 min, 515% buffer B from 1517.5 min, 1535% buffer B from 17.592.5 min, 3595% buffer B from 92.595 min, 95% buffer B from 95105 min, 955% buffer B from 105107 min, and 5% buffer B from 107125 min

The latex is highly caustic and irritating to skin and mucous membranes, while the seeds contain toxalbumins that cause agglutination and hemolysis in RBCs, damage other cell types, and have a lipoid resin complex that can induce dermatitis (670)

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Every recommendation is based on your medical history, current health, wellness goals, and provider evaluation