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Cell debris was removed by centrifugation at 4400 g at 4 C for 30 min and the clarified cell lysate was used for protein purification

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Using mass spectrometry (MS) approach, we then showed that the resulting NCs bind carbonylated proteins through the formation of an oxime bond between the aminooxy-containing thiolated ligand on the NC and the carbonylated amino acid on the protein

It includes professional post-treatment kit Calcium Pyruvate (Medical grade) Calcium pyruvate is a naturally occurring compound that combines calcium with pyruvic acid

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