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The iTRAQ samples were analyzed by using a Velos Orbitrap mass spectrometer (Thermo Scientific), and 18 O-labeled samples were analyzed by using an LTQ-Orbitrap mass spectrometer (Thermo Scientific)

Deparaffinization of the sections was performed using xylene and hydration was performed by passing the sections through changes of ethanol with different concentrations (100% ethanol for 2 min