For maximum precision, switch to a syringe with finer gradations (0.3 mL syringes often have half-unit markings)
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In contrast, DIO mice in both groups switched from WD to LD each had significantly increased Proteobacteria relative abundance (represented solely by the genus Parasutterella ) compared to both the WD and the LD controls, suggesting that Proteobacteria relative abundance may be largely influenced by stress or the differences in net energy expenditure (or associated body weight changes) caused by the diet switch
PDL coating involved adding 100 g ml 1 PDL in sterile H 2 O for 30 min, followed by two washes with H 2 O and air-drying
10.1016/j.brainresbull.2019.05.017 132 XuD.LuY
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