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Following centrifugation, the supernatant was incubated with 50 l Strep-Tactin Sepharose resin, MBP resin, or GST beads (Beyotime, P2253) at 4 C for 1 h

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Extensive analysis of the peptide's stress-protective effects revealed consistent reductions in stress hormone levels and improved stress adaptation across multiple experimental paradigms

However, once an antioxidant gives away its electron, it becomes "spent" or oxidized

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Nutrition and epigenetics: An interplay of dietary methyl donors, one-carbon metabolism and DNA methylation