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Western blots, activity gels and activity assays are various methods used to determine protein and activity in both cells and tissue depending on the amount of protein required for each assay
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This structure mimics your cell walls, allowing for a "friendly" entry into the system
Glutathione can regenerate it
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For experiments testing the effect of AG-120 treatment, media was additionally modified to contain low magnesium concentrations (0.08 mM MgSO 4 ) for 24 hours when indicated, followed by AG-120 (1 M) treatment for 2 hours
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