Initial weight loss in week 1 is usually water weight from reduced food intake and decreased stomach distention, typically 12 pounds
Cells were incubated with serum-free media for 24 h prior to each experiment and seeded at 1 10 6 cells/dish in 100 mm culture dishes for western blotting and 3 10 4 cells/well in 24-well- culture plates for immunofluorescence
The underlying cause, slowed gut motility from receptor activation, produces similar constipation characteristics regardless of which receptors are targeted
Ethics declarations Competing interests The authors declare the following competing interests: SSJ reports consulting relationships with Bristol Myers Squibb, ARTIS Ventures, and Broadview Ventures
Tandon R, Thacker J, Pandya U, Patel M, Tandon K
10.1016/j.ejphar.2020.173288 87 TenrioM.GracilianoN