Immunofluorescent staining Cells were washed with PBS at 1000 rpm for 5 min twice, fixed with 4% paraformaldehyde for 2 h, then permeabilized with 0.1% Triton X-100 for 10 min at room temperature
ferrostatin-1 (depicted in figures as Fer-1) was purchased from Sigma-Aldrich, USA (Cat
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