American Journal of Physiology-Endocrinology and Metabolism, 316 (1), E73-E85
Although glutamine is found naturally in high protein foods, under certain conditions such as stress, chronic inflammation and intense exercise there is an increased demand for glutamine
pNRF2 quantification HCS sections were immunostained with primary anti-pNRF2 antibodies (Abcam, Cambridge, UK, phospho-Ser40, Rabbit monoclonal antibody, Cat# ab76026, RRID: AB_1524049, Dilution 1:200) and Anti-Rabbit Alexa Fluor 647 antibodies (Invitrogen/Fisher Scientific, Goat Polyclonal, Cat# A-21245, RRID: AB_2535813, Dilution: 1:500) as previously described
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Our results further suggest that targeting mitochondrial iron overload or lipid ROS accumulation may represent new cytoprotective strategies for oxidative stress-induced ferroptosis in cardiomyocytes
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