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The sequence used a 2x-150-bp paired-end (PE) configuration

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The pellet from 10 ml of bacterial solution was collected for each sample, resuspended in PBS, and then sonicated for 10 min (200 W total power with 35% output, 2 s pulse, 3 s pause over ice)

Finally, we calculated the ratio of phosphorylated proteins in each pathway and compared them between genotypes

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