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Agarose gels were all 1.5% w/v agarose stained with ethidium bromide (Fischer) and imaged on BioRad GelDoc imager

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Miron VE, Boyd A, Zhao J-W, Yuen TJ, Ruckh JM, Shadrach JL, et al

Using a long-term HFD-fed mouse model of NAFLD, we demonstrated in this study that NAFLD development occurs concomitantly with dysregulated hepatic glutathione homeostasis characterized by decreased GSH and increased GSSG, and increased TNF protein levels in both the circulation and the liver