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Protein was induced with 1 mM IPTG after cells reached an absorbance of 0.5 at 595 nm

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But a follow-up 2021 study, known as STEP 4, showed that within 48 weeks of no longer taking semaglutide, participants regained approximately two-thirds of their prior weight loss, while those who remained on GLP-1 drug therapy continued to lose weight

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