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MALE FERTILITY AND SPERMATOGENESIS Hypogonadotropic Hypogonadism: Steroidogenesis Restoration Serum testosterone: increase from 200 ng/dL above baseline Primary failure: no response (Leydig cell damage) Secondary failure: present response but low baseline Applications: Differentiation between primary and secondary hypogonadism Pre-pubertal Leydig cell reserve evaluation Treatment response prediction 5

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Posted November 25, 2025 in Fertility Blog & Information 18 minute read Key Takeaways Peptides are extremely targeted biological messengers that can impact reproductive hormones and tissue repair

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After fixation of the biopsy specimens, free floating 50-m-thick sections were cut and stained by using rabbit anti-protein gene product 9.5 antibody (Dako Netherlands BV, Eindhoven, the Netherlands) and visualized using a goat anti-rabbit Alexa fluor 488 antibody (Invitrogen,/Life Technologies, Carlsbad, CA, USA)