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proposed the incision located only in the buccal aspect, so it allows to release the periosteum [57]

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Cells were then blocked with 1% bovine serum albumin for 30 min and stained with anti-GFP antibody in PBS containing 1% bovine serum albumin for 1 h

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pH 7.4) containing 1.25% 3,3-diaminobenzidine (DAB: Merck KGaA, Darmstadt, Germany) and 0.75% hydrogen peroxide