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The mean frequency of AMPA-mediated mEPSCs recorded from vehicle-treated neurons was 3.06 0.23 Hz from 33 cells, while Nle 1 -AngIV induced a 1.7-fold increase [5.27 0.43 Hz from 25 cells (mean S.E.M.)

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This limited overlap, particularly when contrasted with broad DUB inhibition, supports the conclusion that age-associated proteome remodeling arises from the coordinated functional decline of multiple cysteine DUBs rather than from the dysfunction of a single enzyme