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NP-40 (600 l, 0.1% in PBS) was added to each sample and transferred to centrifugation columns (Fisher Scientific Pierce) to perform washing by gravity flow

Although anti-CCL2 antibodies have shown promising effects in preclinical studies, their clinical application still requires further optimization to minimize the risk of systemic immunosuppression

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Behavioral analysis following ethanol administration The Laboratory Animal Behaviour Observation, Registration, and Analysis System (LABORAS, Metris B.V., Hoofddorp, Netherlands) was used to investigate rat locomotor movements, following the protocols previously outlined by Castagne et al

J Clin Lab Anal