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4 Discussion Chemically, the indoles are a heterocyclic compound group containing a benzene ring joined to a pyrrole ring, which could be chemically synthesized by different reactions ( Figure 1 ), and different indole analogs are produced such as 3-methylindole (skatole), indoxyl sulfate, indole-3-propionic acid, indole-butyric acid ( The early version of the Salkowski reagent was developed to rapidly determine indole compounds ( -1, and Supplementary Figure S1 , Supplementary Material )

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