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SNIP1 mutations in the FHA domain affect cell viability and pre-mRNA splicing To determine whether mutations in the SNIP1 FHA domain affect incorporation of SNIP1 into the activated spliceosome, we expressed flag-tagged SNIP1 (F-SNIP1) WT, single mutants K265G, K301G, R365G, E366G, and the double mutant K265G/K301G in cells and compared their association with components of the activated spliceosome (Fig

3i) or plasma and tissue PAGly levels (Supplementary Fig

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Weighing in on obesity and psoriatic arthritis - time to move beyond association to robust randomised trials

qRT-PCR was performed as previously reported[14]