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Western blot analysis Proteins were extracted and estimated from the bones samples using RIPA lysis buffer (150 mM NaCl, 0.1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS (sodium dodecyl sulfate), 50 mM TrisHCl, pH was adjusted at 8.0, 0.4 l protease inhibitor buffer, 2.5 l phosphatase inhibitor) from BufferBio BASIC Inc

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[5] Kwon DR, Park GY