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10.1542/peds.2005-2993

Certainly, we suppose that colloidal stabilization for [Ag(GSH)] occur due to the presence of van der waals forces between the negatively partial charges of thiol and acetyl groups that present in the molecular structure of the GSH, with positive charge of Ag-NPs that surround on the surface of NPs

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therefore, tissues should be minced into small pieces and rinsed thoroughly in ice-cold PBS (0.01M, pH 7.4) to remove excess blood