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The supernatant was aspirated, and the free sulfhydryl groups were blocked with 300 L iodoacetamide (IAA) solutioin (130 mM in 25 mM NH 4 HCO 3 ) for 45 min at room temperature in a dark place

148 Similarly, downregulation of the PI3K/AKT pathway inhibits the proliferation, migration, and degranulation of eosinophils, basophils, and mast cells in mice

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Inflammaging decreases adaptive and innate immune responses in mice and humans