Nikiforov, A., Dlle, C., Niere, M
Patients with pre-existing cardiovascular conditions, uncontrolled hypertension, or structural cardiac abnormalities require particularly cautious evaluation, and stimulants may be contraindicated in certain high-risk individuals
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Before each assay appropriate amount of aliquots were thawed, suspended (1 10 5 cells/ml) in assay medium (DMEM w/o phenol red, 10 mM Hepes, 2% ovalbumin and 0.2% Pluronic F-68) and then transferred into a 96 well plate (5 10 3 cells/50 l) followed by addition of ligand and/or Fab 3F52 diluted in assay medium (50 l/well)
z otyoci II stopnia, u ktrych wskanik BMI przekracza 35