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The membrane was incubated at 4C overnight with a primary antibody: anti-IB- (1:1500), anti-NF-B p-65 (1:1000), anti-MMP-9 (1:1000), anti-albumin (1:5000), anti-occludin (1:1500), anti-claudin-5 (1:1000) (Abcam, Cambridge, MA, United States), anti-p-AMPK (1:1000), anti-AMPK (1:1000), anti-p-NF-B p-65 (1:1000) (Cell signaling, Boston, MA, United States), anti-GLP-1R (1:200), and anti--actin (1:5000) (Santa Cruz Biotechnology Inc.)

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Finally, perspectives are given for future directions of selenoprotein research in ASD

Biopsy techniques: diagnosis of melanoma

J Physiol Pharm