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The subsequent treatment of 28 days of saffron (30 mg/kg) or crocin (15 or 30 mg/kg) or saline was performed on the postnatal days 3033 on male offspring

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Samples containing identical amounts of protein were resolved using SDS-PAGE (Criterion, BioRad Laboratories), transferred to nitrocellulose, and blocked in Tris-buffered saline (TBS) containing 5% milk for one hour at room temperature