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Prebiotics, non-digestible fibers that nourish beneficial gut bacteria, also play a crucial role

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AOD9604s mechanism of action remains an active area of research interest

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After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)