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doi: 10.1073/pnas.95.16.9220

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The supernatant was collected from centrifugation at 10,000 g for 15 min at 4 C and 0.1 ml of aliquot was reacted with assay buffer containing 1 mM potassium iodide in 10 mM phosphate-buffered saline (PBS) (pH 7.5)

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