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Complement ELISAs ELISAs were performed by capturing anti-NIP IgG1 variants (3000.023.4 ng/mL) on BSA-NIP(16) (diluted to 1 g/mL in PBS) (Biosearch technologies) coated in 96-well EIA/RIA plates (CorningCostar) or by randomly coating NIP IgG1 mAbs directly in wells (10 g/mL)