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Analysis of apoptosis by propidium iodide staining and flow cytometry

Small (1-3 mm) vesicles (fluid-filled blisters) develop, often clustered together on a bright red or darker-than-normal skin color (hyperpigmented) base

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Artificial additives: Introduce harmful substances that burden liver function

Immunofluorescence staining and small animal live imaging system PKH26 (Millipore Sigma) was used for pre-labeling particles following the manufacturers instruction

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