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Plasmid To construct p4xCRE-3xSRE-TATA-Luc2P, four tandem repeats of the cAMP-response element (5-AGCCTGACGTCAGAG-3) 42,43 , three tandem repeats of the serum-response element (5-AGGATGTCCATATTAGGACATCT-3) 44,45 derived from the human c- fos gene, and an adenovirus E1b TATA sequence (5-AGGGTATATAATG-3) 46,47 were subsequently inserted into a pGL4.11 vector (Promega, Madison, WI, USA)
conducted a network meta-analysis demonstrating that high-dose omega-3 PUFAs were associated with improved clinical outcomes in septic patients, including reduced mortality and organ dysfunction, with favorable safety and acceptability profiles (Tseng et al., 2024)
Environmental stimuli trigger airway epithelial cells to release IL-25, IL-33, and thymic stromal lymphopoietin (TSLP) 4 , which activate T helper (Th)2 cells to produce IL-4, IL-5, and IL-13, driving asthma symptoms
