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Allegheny County recorded 432 fatal overdoses in 2024, down from 665 in 2023, with preliminary 2025 figures showing a further drop

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P values indicated, calculated by one-way ANOVA with Dunnetts multiple comparisons test ( a,c ), two-tailed t -test ( b ) or two-way ANOVA with dks multiple comparisons test

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found that plasma TMAO is positively associated with abundance of 12 bacterial genera in fecal, including three Bacteroidetes (Prevotella 7, Prevotella 2, an uncultured Prevotellaceae)