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The washing process was carried out using 2% acetonitrile in water (with 0.1% formic acid) for 10 min

Additionally, nelfinavir upregulates the expression of CHAC1, a proapoptotic protein and negative regulator of the NOTCH pathway

AOD9604 was originally developed after research on mice, but subsequent studies in human have shown no efficacy for the potential treatment of obesity

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Future research should focus on integrating these biomarkers into clinical practice to improve diagnostic accuracy and treatment outcomes