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Blots were blocked for 1 h in 5% non-fat milk in phosphate-buffered saline (PBS) with 0.05% Tween-20, and membranes were probed with primary antibodies overnight and horseradish-peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA, USA)

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He was released a week later

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This essential nutrient is crucial for red blood cell production, a healthy nervous system, and DNA synthesis