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The isolated mRNA was reverse transcribed via the Superscript III First-Strand Synthesis System (ThermoFisher Scientific) according to manufacturers instructions

Front Physiol (2018) 9:113

40 In a clinical study by Rajasekaran et al., the anatomy of adult lumbar disc herniation was investigated to document the site of disc failure using different image modalities and clinical, as well histological, examinations of surgically retrieved disc specimens

Same syringe marking, different dose

When contamination is adequately controlled, non-Lactobacillus-dominant profiles have been associated with increased risk of implantation failure and may contribute to the inflammatory endometrial phenotypes observed in a subset of RIF and RM patients [380]

Taking only the required amount reduces this risk