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then, 400 l of extraction solution (methanol:acetonitrile = 1:1) was added before vortexing and mixing for 30 s, followed by ultrasonication for 10 min in an ice-water bath

The biggest thing is not overthinking it

422 Some studies also showed that HER2 heterogeneity correlated with worse clinical outcomes, 423 and HER2 intra-tumoral heterogeneity could be another potential factor for the resistance to anti-HER2 therapy

This is because their bodies dont absorb it as well

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