After centrifugation (250 x g , 5 min, 4C), cells were washed with HBS and measured using flow cytometry (FACSCantoII, BD Biosciences, San Jose, CA, USA)
This shift toward MC4R selectivity makes PT141 a useful tool for isolating MC4Rmediated signalling including Gproteincoupled cAMP and phospholipaseC cascades, intracellular calcium flux, and ERK/MAPK pathway activation within neuronal and receptorexpressing model systems
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C-Tetra Intense Squalane, Simmondsia Chinensis (Jojoba) Seed Oil, Tetrahexyldecyl Ascorbate, Dimethicone, Candida Bombicola/Glucose/Methyl Rapeseedate Ferment, Citrus Grandis (Grapefruit) Peel Oil, Ethyl Linoleate, Passiflora Edulis (Maracuja) Seed Oil, Tocopheryl Acetate, Panax Ginseng Root Oil, Olea Europaea (Olive) Fruit Oil, Helianthus Annuus (Sunflower) Seed Oil, Prunus Armeniaca (Apricot) Kernel Oil, Prunus Amygdalus Dulcis (Sweet Almond) Oil, PPG-12/SMDI Copolymer, Limonene
Before using an injection, read the drug recommendations and follow all directions on the prescription label
Hsiao, E