2.7 RNA extraction and quantitative real-time PCR (qRT-PCR) Total RNA was extracted from PIG1 and PIG3V cells using TRIzol reagent (#15596018, Invitrogen, USA), and its concentration and purity were assessed using a NanoDrop 2000 device (Thermo Fisher, USA)
Four weeks after the initiation of antibiotics, nave, resolved and sensitized mice were used to isolate primary USCs or used for secondary infection assay
51 OkinoS
Health History Questionnaire: You will answer questions about your medical history, travel, and lifestyle
However, long-term use can lead to significant side effects, including: Disrupted gut health
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