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Therefore, the observed reduction in the severity of symptoms as assessed by the SFNSL cannot be attributed with certainty only to effects of ARA 290 on small fiber function

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Next, membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% (v/v) Tween 20 (TBST) for 1 h at room temperature and probed with primary antibodies against corresponding antigens overnight at 4 C