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Marx W, McGuinness AJ, Rocks T, Ruusunen A, Cleminson J, Walker AJ, et al

All roGFP2fusion proteins used in this study were constitutively expressed from the low copynumber ( CEN ) p415 ( LEU2 marker) or p416 plasmids ( URA3 marker) under the control of a constitutive TEF promoter (from translation elongation factor 1 gene, TEF2, yeast)

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However, if your appetite is very low, even 20 to 25 grams per meal is a win