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Defective mitochondria produce large quantities of oxygen free radicals

Another mechanism involves the oxidative inactivation of cyclic guanosine monophosphate (cGMP), which mediates the effects of NO on sleep homeostasis, and the upregulation of NF-kB by inducible nitric oxide synthase (iNOS) from activated microglia, significantly influencing sleep homeostasis (Cespuglio et al

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Consequently, the accumulated NAPQI binds covalently to the cysteine, methionine, tyrosine, and tryptophan residues of various proteins, forming NAPQI-protein adducts within the cell that disrupt normal physiological protein functions

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