Researchers have investigated this compound as a tool for modulating NNMT activity in in vitro and preclinical models, providing insight into the enzyme's role in metabolic homeostasis
Their provider wants to try tirzepatide
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10.1093/jnci/92.12.977 J
Beads were initially equilibrated in 20 mM Tris-HCl pH 8.0, 300 mM NaCl binding buffer, followed by incubation with 50 M His-tagged Syn constructs (Syn WT or Syn 1103 ) for 45 min at 4 C with gentle rotation
Membranes were probed using antibodies to FoxO1 (1:2500, 18592-1AP, Proteintech, Chicago, IL, USA), SOD2 (1:6000, 66474-1lg, Proteintech, Chicago, IL, USA), NOX4 (1:600, 14347-1-AP, Proteintech, Chicago, IL, USA), and -actin (1:2000, AM10218-400, Abgent) as the primary antibodies