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In short, the proximal small intestine was flushed with ice cold PBS, opened longitudinally, cut into small pieces and transferred to HBSS (Gibco, 14175-053) containing 10% FCS, DTT (1 mM) and EDTA (2 mM) followed by shaking for 20 min at room temperature

Research has shown that temperature cyclingrepeatedly moving a solution from 4C to room temperaturecan cause a gradual loss in GIP/GLP-1/GCG receptor activation potency

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Over another four weeks, the dose might go up to 1.0 mg, continuing the gradual dose escalation

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If an individual has Type 2 diabetes and is overweight or has obesity, the dosage may be the same