The signal intensity, which is a measure of superoxide formation, was analyzed after taking images with a live cell fluorescent microscope (Leica Microsystems, Concord ON, Canada) with 40 magnification
Keep upright, protected from light
Inject bacteriostatic water slowly down the vial wall, not directly onto the lyophilised powder, to prevent foaming
For instance, while some trials use standardized doses over controlled periods, others vary considerably in their protocols, making it difficult to compare their outcomes 51 directly
Dosing and benefit summary Many of ALAs studied effects appear to be dose-dependent, with stronger responses seen at higher doses
Annu Rev Plant Biol 64:161187 Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase