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2013 Jul 21;6(Suppl 1):39-45

& Sohal, J

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Immunoblotting and immunofluorescence staining Gel electrophoresis: unless otherwise noted, SDS-PAGE was run with self-made 12% Tris-glycine SDS-polyacrylamide gel in Tris-glycine SDS running buffer (25 mM Tris, 0.2 M glycine, 0.1% SDS) at 90 V for 15 min stacking and followed by 120 V until sufficient separation was visualised by PageRuler pre-stained protein ladder

Platelet-rich plasma (PRP) therapy, for example, provides a mixture of autologous growth factors and cytokines and has more established clinical evidence for certain applications

The GSH binding site is highly conserved in enteroviruses The key interacting residues in the GSH-binding site are conserved across a panel of representative enteroviruses, including the three serotypes of PV and EVF3 30 (Fig