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After 24 h transfection, cells were resuspended with ice-cold GST-lysis buffer (20 mM Tris-HCl, pH 8.0, 1% Triton X-100 and 10% glycerol in PBS) supplemented with a protease inhibitor cocktail (Roche) and 50 M Palmostatin B (for SwissKASH-derived samples), and passed 10 times through a 25 G needle

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Conclusions The MPNST treatment philosophy can therefore be summarized as thus: maximal surgical resection, potentially in combination with neoadjuvant chemotherapy or radiotherapy to render an initially inoperable tumor operable, followed by adjuvant chemotherapy or radiotherapy when the margins of resection are positive or metastatic disease exists