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That side-chain modification is done chemically after purifying the underlying peptide from the fermentation step, and you can be sure that that process, along with the yeast expression DNA sequence, the fermentation conditions, and the purification steps have been pretty well tuned up by the Novo Nordisk folks

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Actual production costs may vary depending on manufacturing scale, production conditions, and procurement arrangements, while trade shipment prices to and from India reflect current market conditions rather than the minimum production costs that could be achieved through large-scale generic semaglutide manufacturing

PMID: 26029106