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All immunofluorescence images were obtained using an LMS700 laser scanning confocal microscope (Zeiss, Oberkochen, Germany), and the images were analyzed using Image-Pro plus 6.0 software (Media Cybernetics, Silver Spring, MD, USA)

10.1007/s12035-012-8326-1 Mol

Nature 518 , 417421 (2015)

The pathophysiology is intricate, multifaceted, and consequently poorly elucidated

Visible results typically appear within 6-8 weeks of consistent daily use

Metabolic pathway analysis The MetaboAnalyst 6.0 software was used for the analysis of metabolic pathways based on the significant metabolites identified through prior supervised OPLS-DA models